pco.flim 熒光壽命成像相機作為第一款采用每像素兩個讀出抽頭（Two-Tap）CMOS傳感器的頻域(FD) FLIM相機，我們的pco.flim提供像素和激發光的同步調制，從而能夠分析亞納秒到微秒范圍內的發光衰減時間。憑借1008 x 1008的像素分辨率，pco.flim最大可讀出45雙幀/秒，并可用于5 kHz - 40 MHz的調制頻率范圍。這款相機兼具靈活耦合和簡便設置，為廣角FD-FLIM儀器的理想選擇。pco.flim相機系統可通過C接口靈活耦合至任何顯微鏡或鏡頭，并設有一個USB 3.0接口，可輕松將相機連接至各種計算機。pco.flim激光是一種均勻照明的光源，設計用于pco.flim。其數字調制頻率范圍為0 - 250 MHz。pco.flim激光適用于寬場落射熒光顯微鏡照明、全內反射熒光（TIRF）顯微鏡、光片熒光顯微鏡（LSFM）和共聚焦轉盤顯微鏡。各種不同的波長和光輸出功率任您選擇。pco.flim激光非常適合用于亞納秒到微秒范圍內的相關發光壽命樣本。對于使用尼康微鏡的用戶，另提供尼康雙安全快門選擇。pco.flim相機系統是采用Two-Tap CMOS成像芯片的熒光壽命成像相機。相機的像素和激發光間的同步使100皮秒至100微秒間的熒光衰減時間的分析成為可能。其多種觸發選項使相機可以方便的集成于多種實際應用。

德國PCO公司首次發布了這款采用Two-Tap CMOS技術的熒光壽命成像系統Flim，應用范圍涵蓋分子特性分析和測試，FRET，離子濃度/PH值測量，生物組織學/病理學，壓感材料等，可廣泛應用于生命科學、物理科學，材料科學領域。

主要特點：

• 100 ps到100μs的壽命測量

• 調制頻率從5千赫茲到40兆赫

• 500千赫茲到40兆赫外部調制信號

• 正弦/矩形的調制信號波形

• 像素分辨率1008 x 1008

• 頻域FLIM

• USB 3.0接口

• 量子效率39%

• 動態范圍1000:1

• 讀出噪音48 e- rms

• 90幀每秒 (2 tap讀出)

• 可選曝光時間為10ns到10秒

• 無振動水冷

• 特殊的測量與分析軟件

技術參數：

應用案例：

Figure 1: The left photo shows the fluorescence intensity of HEK-293 cells which expressed a CFP/DJ-1 protein as control of a FRET experiment. The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 – 4 ns (imageJ LUT 16 colors, colorbar 0 – 4 ns) which has been masked by an intensity filter. The range of lifetimes around 2 ns was found in all of the 26 cells, which have been measured and showed about 10% FRET efficiency compared to the pure CFP expression. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded using the same colorbar and LUT) without mask (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).

Figure 5:The left image shows the fluorescence intensity of a sliced daisy sample (20x air objective). The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 - 4 ns (imageJ LUT 16 colors, colorbar 0-4 ns). The difference in fluorescence lifetimes clearly allows to differentiate the cells and the pollen grains. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded, the modulation frequency was 30 MHz) (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).

注：產品信息若有變更恕不另行通知